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Table 1 The primers used for cloning P450 genes, qRT-PCR reactions, and SNP determinations

From: Co-up-regulation of three P450 genes in response to permethrin exposure in permethrin resistant house flies, Musca domestica

Gene Primer Name Function Primer Sequence Position (nt)
CYP4G2 AP450HF20F Full length cloning 5' ATGGATAGTGCAAACAATTCAACTGCG 3' 1 to 27
  SNP450HF20F SNaP determination 5' AAATGGCCATTTGGTGGCCCAT 3' 174 to 195
  RTP450HF20F qRT-PCR 5' CGAGGAGGATGATGAAATAAGCAAGC 3' 837 to 862
  RTP450HF20R qRT-PCR 5' TTGGACATGGCCATCATGGCATCT 3' 957 to 980
  P450HF20R 5'-RACE 5' GCAGGAAGTTGTCACCAAAGATG 3' 1140 to 1162
  P450HF20F 3'-RACE 5' CGTGCATCGCAATCCCCAATAC 3' 1341 to 1362
  AP450HF20R Full length cloning 5' TTACATAGCTTTCATGGCTTCGGGTC 3' 1625 to 1650
CYP4D4v2 AP450HF5F Full length cloning 5' ATGTTATTTGAATTCCTGGTGGGTC 3' 1 to 25
  SNP450HF5F SNaP determination 5' ACATGACACCACGACAAGTGG 3' 948 to 968
  RTP450HF5F qRT-PCR 5' AGGATAAGGAGAAACCGGTGACC 3' 1052 to 1074
  RTP450HF5R qRT-PCR 5' CAATTGTCGGCACCGATGGATAC 3' 1156 to 1134
  P450HF5R 5'-RACE 5' AGCAACTCAAAATGGCGTACC 3' 1430 to 1410
  AP450HF5R Full length cloning 5' TAACTACTTGCGAACTCTCAAACCC 3' 1521 to 1497
CYP6A38 P450HF17P-F 5' flanking region cloning 5' TGGTCTTCTAGGGGAGAAGACTACCTGC 3' -676 to -649
  SNP450HF17PF SNaP determination 5' TTCAGGATTGCTGGGTAGCT 3' -69 to -50
  P450HF16F-3 Full length cloning 5' CATTATGGAGACTTCGGGAGTTTTG 3' -4 to 21
  P450HF17P 5' flanking region cloning 5' CGTAGGTTCCTCATGGGGTATACCCAGC 3' 120 to 93
  RTP450HF17F qRT-PCR 5' CCCTGATGGGCAACATGAATGGAT 3' 122 to 145
  RTP450HF17R qRT-PCR 5' TAGTTGTTTGTCCAGCAGCACCAC 3' 270 to 247
  P450HF17R 5' RACE 5' CGCTGTACTTCAATAGATTTCCTGC 3' 650 to 626
  P450HF17F 3' RACE 5' TGAGGGTGATACAAAACCAAGC 3' 1017 to 1038
  AP450HF17R Full length cloning 5' GAGATAATCTCCCACCCCTAAATCG 3' 1520 to 1496
Common Flyh1   5' GGICCIAGIAACTGCATIGG 3'  
  Flyc1   5' GGAAGTNGACACNTTYATGTT 3'  
  CYP6AD1   5' GTNATHGGHHNBTGYGCHTTYGG 3'  
  HemeR1   5' CCIATGCAGTTICTIGGICC 3'  
  Oligo (dt)   5' TAATACGACTCACTATAGGGAGATTTTTTTTTTTTTTTT 3'  
  C2   5' TAATACGACTCACTATAGGGAGA 3'  
  AP1 (RACE)   5' CCATCCTAATACGACTCACTATAGGGC 3'  
  AP1 (GenomeWalking)   5' GTAATACGACTCACTATAGGGC 3'  
  ActinS1   5' AGGCGAATCGCGAGAAGATG 3'  
  ActinAS1   5' TCAGATCACGACCAGCCAGATC 3'  
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BMC Physiology

ISSN: 1472-6793

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